NoPv1: a synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight.
Identifieur interne : 000106 ( Main/Exploration ); précédent : 000105; suivant : 000107NoPv1: a synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight.
Auteurs : Monica Colombo [Italie] ; Simona Masiero [Italie] ; Stefano Rosa [Italie] ; Elisabetta Caporali [Italie] ; Silvia Laura Toffolatti [Italie] ; Chiara Mizzotti [Italie] ; Luca Tadini [Italie] ; Fabio Rossi [Italie] ; Sara Pellegrino [Italie] ; Rita Musetti [Italie] ; Riccardo Velasco [Italie] ; Michele Perazzolli [Italie] ; Silvia Vezzulli [Italie] ; Paolo Pesaresi [Italie]Source :
- Scientific reports [ 2045-2322 ] ; 2020.
Abstract
Grapevine (Vitis vinifera L.) is a crop of major economic importance. However, grapevine yield is guaranteed by the massive use of pesticides to counteract pathogen infections. Under temperate-humid climate conditions, downy mildew is a primary threat for viticulture. Downy mildew is caused by the biotrophic oomycete Plasmopara viticola Berl. & de Toni, which can attack grapevine green tissues. In lack of treatments and with favourable weather conditions, downy mildew can devastate up to 75% of grape cultivation in one season and weaken newly born shoots, causing serious economic losses. Nevertheless, the repeated and massive use of some fungicides can lead to environmental pollution, negative impact on non-targeted organisms, development of resistance, residual toxicity and can foster human health concerns. In this manuscript, we provide an innovative approach to obtain specific pathogen protection for plants. By using the yeast two-hybrid approach and the P. viticola cellulose synthase 2 (PvCesA2), as target enzyme, we screened a combinatorial 8 amino acid peptide library with the aim to identify interacting peptides, potentially able to inhibit PvCesa2. Here, we demonstrate that the NoPv1 peptide aptamer prevents P. viticola germ tube formation and grapevine leaf infection without affecting the growth of non-target organisms and without being toxic for human cells. Furthermore, NoPv1 is also able to counteract Phytophthora infestans growth, the causal agent of late blight in potato and tomato, possibly as a consequence of the high amino acid sequence similarity between P. viticola and P. infestans cellulose synthase enzymes.
DOI: 10.1038/s41598-020-73027-x
PubMed: 33067553
PubMed Central: PMC7567880
Affiliations:
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grapevine downy mildew and potato late blight.</title><author><name sortKey="Colombo, Monica" sort="Colombo, Monica" uniqKey="Colombo M" first="Monica" last="Colombo">Monica Colombo</name><affiliation wicri:level="1"><nlm:affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige</wicri:regionArea><wicri:noRegion>San Michele all'Adige</wicri:noRegion></affiliation></author><author><name sortKey="Masiero, Simona" sort="Masiero, Simona" uniqKey="Masiero S" first="Simona" last="Masiero">Simona Masiero</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Rosa, Stefano" sort="Rosa, Stefano" uniqKey="Rosa S" first="Stefano" last="Rosa">Stefano Rosa</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Caporali, Elisabetta" sort="Caporali, Elisabetta" uniqKey="Caporali E" first="Elisabetta" last="Caporali">Elisabetta Caporali</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Toffolatti, Silvia Laura" sort="Toffolatti, Silvia Laura" uniqKey="Toffolatti S" first="Silvia Laura" last="Toffolatti">Silvia Laura Toffolatti</name><affiliation wicri:level="3"><nlm:affiliation>Department of Agricultural and Environmental Sciences (DISAA), University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural and Environmental Sciences (DISAA), University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Mizzotti, Chiara" sort="Mizzotti, Chiara" uniqKey="Mizzotti C" first="Chiara" last="Mizzotti">Chiara Mizzotti</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Tadini, Luca" sort="Tadini, Luca" uniqKey="Tadini L" first="Luca" last="Tadini">Luca Tadini</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Rossi, Fabio" sort="Rossi, Fabio" uniqKey="Rossi F" first="Fabio" last="Rossi">Fabio Rossi</name><affiliation wicri:level="3"><nlm:affiliation>Center for Study and Research on Obesity, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Center for Study and Research on Obesity, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Pellegrino, Sara" sort="Pellegrino, Sara" uniqKey="Pellegrino S" first="Sara" last="Pellegrino">Sara Pellegrino</name><affiliation wicri:level="3"><nlm:affiliation>DISFARM-Department of Pharmaceutical Sciences, University of Milan, Milan, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>DISFARM-Department of Pharmaceutical Sciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Musetti, Rita" sort="Musetti, Rita" uniqKey="Musetti R" first="Rita" last="Musetti">Rita Musetti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine</wicri:regionArea><wicri:noRegion>Udine</wicri:noRegion></affiliation></author><author><name sortKey="Velasco, Riccardo" sort="Velasco, Riccardo" uniqKey="Velasco R" first="Riccardo" last="Velasco">Riccardo Velasco</name><affiliation wicri:level="1"><nlm:affiliation>CREA Research Centre for Viticulture and Enology, Conegliano, TV, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>CREA Research Centre for Viticulture and Enology, Conegliano, TV</wicri:regionArea><wicri:noRegion>TV</wicri:noRegion></affiliation></author><author><name sortKey="Perazzolli, Michele" sort="Perazzolli, Michele" uniqKey="Perazzolli M" first="Michele" last="Perazzolli">Michele Perazzolli</name><affiliation wicri:level="1"><nlm:affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige</wicri:regionArea><wicri:noRegion>San Michele all'Adige</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Centre Agriculture Food Environment (C3A), University of Trento, San Michele all'Adige, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Centre Agriculture Food Environment (C3A), University of Trento, San Michele all'Adige</wicri:regionArea><wicri:noRegion>San Michele all'Adige</wicri:noRegion></affiliation></author><author><name sortKey="Vezzulli, Silvia" sort="Vezzulli, Silvia" uniqKey="Vezzulli S" first="Silvia" last="Vezzulli">Silvia Vezzulli</name><affiliation wicri:level="1"><nlm:affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy. silvia.vezzulli@fmach.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige</wicri:regionArea><wicri:noRegion>San Michele all'Adige</wicri:noRegion></affiliation></author><author><name sortKey="Pesaresi, Paolo" sort="Pesaresi, Paolo" uniqKey="Pesaresi P" first="Paolo" last="Pesaresi">Paolo Pesaresi</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biosciences, University of Milan, Milan, Italy. paolo.pesaresi@unimi.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biosciences, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Grapevine (Vitis vinifera L.) is a crop of major economic importance. However, grapevine yield is guaranteed by the massive use of pesticides to counteract pathogen infections. Under temperate-humid climate conditions, downy mildew is a primary threat for viticulture. Downy mildew is caused by the biotrophic oomycete Plasmopara viticola Berl. & de Toni, which can attack grapevine green tissues. In lack of treatments and with favourable weather conditions, downy mildew can devastate up to 75% of grape cultivation in one season and weaken newly born shoots, causing serious economic losses. Nevertheless, the repeated and massive use of some fungicides can lead to environmental pollution, negative impact on non-targeted organisms, development of resistance, residual toxicity and can foster human health concerns. In this manuscript, we provide an innovative approach to obtain specific pathogen protection for plants. By using the yeast two-hybrid approach and the P. viticola cellulose synthase 2 (PvCesA2), as target enzyme, we screened a combinatorial 8 amino acid peptide library with the aim to identify interacting peptides, potentially able to inhibit PvCesa2. Here, we demonstrate that the NoPv1 peptide aptamer prevents P. viticola germ tube formation and grapevine leaf infection without affecting the growth of non-target organisms and without being toxic for human cells. Furthermore, NoPv1 is also able to counteract Phytophthora infestans growth, the causal agent of late blight in potato and tomato, possibly as a consequence of the high amino acid sequence similarity between P. viticola and P. infestans cellulose synthase enzymes.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33067553</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>10</Month><Day>16</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>NoPv1: a synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight.</ArticleTitle><Pagination><MedlinePgn>17574</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-020-73027-x</ELocationID><Abstract><AbstractText>Grapevine (Vitis vinifera L.) is a crop of major economic importance. However, grapevine yield is guaranteed by the massive use of pesticides to counteract pathogen infections. Under temperate-humid climate conditions, downy mildew is a primary threat for viticulture. Downy mildew is caused by the biotrophic oomycete Plasmopara viticola Berl. & de Toni, which can attack grapevine green tissues. In lack of treatments and with favourable weather conditions, downy mildew can devastate up to 75% of grape cultivation in one season and weaken newly born shoots, causing serious economic losses. Nevertheless, the repeated and massive use of some fungicides can lead to environmental pollution, negative impact on non-targeted organisms, development of resistance, residual toxicity and can foster human health concerns. In this manuscript, we provide an innovative approach to obtain specific pathogen protection for plants. By using the yeast two-hybrid approach and the P. viticola cellulose synthase 2 (PvCesA2), as target enzyme, we screened a combinatorial 8 amino acid peptide library with the aim to identify interacting peptides, potentially able to inhibit PvCesa2. Here, we demonstrate that the NoPv1 peptide aptamer prevents P. viticola germ tube formation and grapevine leaf infection without affecting the growth of non-target organisms and without being toxic for human cells. Furthermore, NoPv1 is also able to counteract Phytophthora infestans growth, the causal agent of late blight in potato and tomato, possibly as a consequence of the high amino acid sequence similarity between P. viticola and P. infestans cellulose synthase enzymes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Colombo</LastName><ForeName>Monica</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masiero</LastName><ForeName>Simona</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosa</LastName><ForeName>Stefano</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Caporali</LastName><ForeName>Elisabetta</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Toffolatti</LastName><ForeName>Silvia Laura</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Department of Agricultural and Environmental Sciences (DISAA), University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mizzotti</LastName><ForeName>Chiara</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tadini</LastName><ForeName>Luca</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rossi</LastName><ForeName>Fabio</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Center for Study and Research on Obesity, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pellegrino</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>DISFARM-Department of Pharmaceutical Sciences, University of Milan, Milan, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Musetti</LastName><ForeName>Rita</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Velasco</LastName><ForeName>Riccardo</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>CREA Research Centre for Viticulture and Enology, Conegliano, TV, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Perazzolli</LastName><ForeName>Michele</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre Agriculture Food Environment (C3A), University of Trento, San Michele all'Adige, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vezzulli</LastName><ForeName>Silvia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy. silvia.vezzulli@fmach.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pesaresi</LastName><ForeName>Paolo</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Biosciences, University of Milan, Milan, Italy. paolo.pesaresi@unimi.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>14</Day></PubMedPubDate><PubMedPubDate 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